Lethality of embryos inoculated with Y. enterocolitica strains ( flhB mutant). The ELA was performed with Y. enterocolitica 8081v and its isogenic flhB mutant. Control groups of embryos were either uninoculated or inoculated with PBS. Embryos were inoculated with 108 CFU of the Y. enterocolitica strains. Lethality of the infected embryos was determined for five consecutive days. The experiment was performed three times. The Figure combines the number of dead embryos across the three experiments for each inoculum. Data are expressed as percentage of the total number of embryos for that inoculum. White capital letters in the Y. enterocolitica data bars represent the different groups from the Duncan's multiple group comparison.