Figure 8

Time-course of DENV infection in Ae. aegypti midgut. Viral envelope antigen distribution during the DENV infection was analyzed in the MG of the DS3 strain (A); DMEB strain (B); or IBO-11 strain (C) in the Cardia/AMG (anterior midgut), aPMG (anterior segment of the posterior midgut), pPMG (posterior segment of the posterior midgut). Values are expressed as the mean ± S.E.M. ANOVA followed by All Pairwise Multiple Comparison Procedures (Student-Newman-Keuls Method) (p < 0.05); n = 4 independent replications included at least 5 mosquitoes for each strain and each infection time point. Values from DS3 or DMEB strains were compared with IBO-11 strain at 13, 26 and 336 h in each MG region (*p < 0.05, significantly different); Cardia/AMG, aPMG, or pPMG values from DMEB, DS3 or IBO-11 strains at 5 h post-infection were compared with those post-infected for 13, 26 and 336 h in the respectively strains (**p < 0.05, significantly different; ^#p > 0.05, non-significantly different).