Figure 3From: Processing of predicted substrates of fungal Kex2 proteinases from Candida albicans, C. glabrata, Saccharomyces cerevisiae and Pichia pastorisActivity testing of refolded CaPho11. CaPho11 was isolated from denatured inclusion bodies and refolded. To ensure its correct folding, activity testing using pNPP as a substrate was performed at different pH values. The enzyme was active and exhibited a maximum activity at pH 4.2–4.3.Back to article page