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Table 4 Primers used in this study

From: Development of methods for the genetic manipulation of Flavobacterium columnare

Primer Sequencea
pr26 5'-GCTAGGTACCATTTTTACTTTTTAGTGTTTCTATAAAAG-3'
pr32 5'-CCCGAAGCAGGGTTATGCAGCGGAAAAATT-3'
pr33 5'-GCCGATTGCCGACTGGTTCAGGGAGCAAT-3'
pr35 5'-GCTAGGTACCTCGAGCCTGTACCCATAAGATTAATACTAAATAA-3'
pr37 5'-GCTAGGTACCATGAGTAAAGGAGAAGAACTTTTCAC-3'
pr38 5'-GCTAGCTGCAGCAGATCTATTTGTATAGTTCATCCA-3'
pr44 5'-GGTACCGGCAGCGCATACCAAAGAACACTTAGACAAGGCA-3'
pr45 5'-GCTAGGTACCTTTTTAATTACAATTTAGTTAATTACAAGCAAAA-3'
pr46 5'-GCTAGCCCGGGCACGATTGGAATAACACTCCATCTCAGC-3'
pr47 5'-GCTAGCATGCACCTACGCGAGACATAGCACATCT-3'
pr54 5'-TTGGTGGTGGACCCGTTG-3'
pr55 5'-GCTGTTTCACTCGGTTTATTCTCA-3'
pr56 5'-ATCACCTTCACCCTCTCCACTGAC-3'
pr88 5'-TTAATGCAGCTGGCACGACAGGTT-3'
pr93 5'-AAACATTTCCCTCCTTAT-3'
pr104 5'-ACCTACTGAAAGTATGAAAGTAAAC-3'
  1. arestriction sites on primers are underlined