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Table 3 Media used in this study

From: Development of methods for the genetic manipulation of Flavobacterium columnare

Ingredients (g L-1) Ordals/Cytophaga (Ord)a Modifled Ordals (MO)b FCGMc
Tryptone 0.5 0.5 8.0
Beef extract 0.2 0.2  
Yeast extract 0.5 0.5 0.8
NaCl   1.76d 5.0
Na2SO4   0.147d  
NaHCO3   0.008d  
KCl   0.025d  
KBr   0.004d  
MgCl2 × 6 H2O   0.187d  
MgSO4 × 7 H2O    1.0
CaCl2 × 2 H2O   0.041d 0.74
SrCl2 × 6 H2O   0.0008d  
H3BO3   0.0008d  
Sodium acetate 0.2 0.2  
Sodium citrate    1.5
Agar (for plates) 10 10 10
  1. aOrdal and Rucker [54]
  2. bThis study. c Farmer [30]
  3. dSalts were mixed in a 10× stock as NSS after Marden et al. [51]