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Table 2 Strains and plasmids used in this study

From: Development of methods for the genetic manipulation of Flavobacterium columnare

Strain or plasmid Genotype or description Source or reference
Bacterial Strains   
   E. coli   
S17-1 hsdR17 (rk- mk-)recA RP4-2(Tcr::Mu-Kmr::Tn7 Strr)  
TOP10 F- mcrA Δ(mrr-hsdRMA-mcrBC) φ80lacΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG Invitrogen
   F. columnare   
C#2 Wild Type [13]
AL-203-94 Wild Type [13]
Fc14-56 Wild Type [26]
94-060 Wild Type [26]
1191-B Wild Type [26]
94-078 Wild Type [26]
94-081 Wild Type [26]
90-059 Wild Type [26]
L90-659 Wild Type [26]
92-002 Wild Type [26]
C91-20 Wild Type [26]
FcAS44 gldJ knockout mutant of C#2 This Study
Plasmids   
   pAMSTA39 PCR cloning vector with promoter-less gfp; Apr Kmr This Study
   pAS29 Promoter-less gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr) This Study
   pAS36 Pmap-gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr) This Study
   pAS42 1400-bp fragment of gldJ in pCP29; Apr (Emr, Cfr) This Study
   pAS43 PompA-gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr) This Study
   pCE320 gfp-containing E. coli-Borrelia burgdorferi shuttle vector; Apr [46]
   pCR4-TOPO PCR cloning vector; Apr Kmr Invitrogen
   pCP11 E. coli – Flavobacterium shuttle plasmid; Apr (Emr) [21]
   pCP29 E. coli – Flavobacterium shuttle plasmid; Apr (Cfr Emr) [37]
   pEP4351 λpir dependent R6K oriV; RP4 oriT; Cmr Tcr (Emr); Tn4351 mutagenesis vector [53]
   pCR4-TOPO PCR cloning vector; Apr Kmr Invitrogen