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Table 2 Strains and plasmids used in this study

From: Development of methods for the genetic manipulation of Flavobacterium columnare

Strain or plasmid

Genotype or description

Source or reference

Bacterial Strains

  

   E. coli

  

S17-1

hsdR17 (rk- mk-)recA RP4-2(Tcr::Mu-Kmr::Tn7 Strr)

 

TOP10

F- mcrA Δ(mrr-hsdRMA-mcrBC) φ80lacΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG

Invitrogen

   F. columnare

  

C#2

Wild Type

[13]

AL-203-94

Wild Type

[13]

Fc14-56

Wild Type

[26]

94-060

Wild Type

[26]

1191-B

Wild Type

[26]

94-078

Wild Type

[26]

94-081

Wild Type

[26]

90-059

Wild Type

[26]

L90-659

Wild Type

[26]

92-002

Wild Type

[26]

C91-20

Wild Type

[26]

FcAS44

gldJ knockout mutant of C#2

This Study

Plasmids

  

   pAMSTA39

PCR cloning vector with promoter-less gfp; Apr Kmr

This Study

   pAS29

Promoter-less gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr)

This Study

   pAS36

Pmap-gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr)

This Study

   pAS42

1400-bp fragment of gldJ in pCP29; Apr (Emr, Cfr)

This Study

   pAS43

PompA-gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr)

This Study

   pCE320

gfp-containing E. coli-Borrelia burgdorferi shuttle vector; Apr

[46]

   pCR4-TOPO

PCR cloning vector; Apr Kmr

Invitrogen

   pCP11

E. coli – Flavobacterium shuttle plasmid; Apr (Emr)

[21]

   pCP29

E. coli – Flavobacterium shuttle plasmid; Apr (Cfr Emr)

[37]

   pEP4351

λpir dependent R6K oriV; RP4 oriT; Cmr Tcr (Emr); Tn4351 mutagenesis vector

[53]

   pCR4-TOPO

PCR cloning vector; Apr Kmr

Invitrogen