Figure 2
From: Development of methods for the genetic manipulation of Flavobacterium columnare
Demonstration of Tn 4351 transposable element integration into the genome of F. columnare strain AL-203-94 following conjugative mating. Primers pr54 and pr56 targeting a 435 bp fragment of the tetX gene contained within the transposon were used to screen for the presence of the transposon in F. columnare genomic DNA. PCR products were run on a 1% agarose gel at 80 V for 45 min and visualized after staining with ethidium bromide. Lane 1: 1 kb ladder, markers range from 250 to 10,000 bp; Lanes 2–11: PCR product from genomic DNA extracted from colonies that grew on an Ordals agar plate augmented with 1 μg ml-1 of erythromycin and Lane 12: Tn4351 containing plasmid pEP4351 (positive control).