Figure 1

Demonstration of Gfp expression levels in F. columnare strain C#2 containing plasmids pAS29 (A and B) pAS36 (C and D) and pAS43 (E and F) using epifluorescence (A, C and E) and transillumination/phase contrast (B, D and F) microscopy. The same field is shown for epifluorescence and phase contrast micrographs for each strain. Exposure was varied in the pictures using transillumination to optimize each image, but for comparative purposes the excitation energy and image exposure times were held constant in the three epifluorescence images. All six panels are drawn to the same scale.