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Figure 7 | BMC Microbiology

Figure 7

From: Gene disruption of the DNA topoisomerase IB small subunit induces a non-viable phenotype in the hemoflagellate Leishmania major

Figure 7

Analysis of DNA content in L. major promastigotes after reversible arrest with HU. Cells obtained from 12 h-arrest with 5 mM HU (time 0) were harvested and incubated in fresh media in presence or absence of 5 μM CPT. After the time periods indicated, cells were stained with propidium iodide (PI) and analyzed by flow-cytometry. The graphs represent the relative PI fluorescence plotted against the frequency of events per channel. Each plot represents data collected from 20,000 events/sample.

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BMC Microbiology

ISSN: 1471-2180

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