Figure 2

Expression of LmTopIB under different genetic manipulations A) Western analysis measuring TopIL protein abundance of WT, LmTopIB-overexpressing and (ΔtopS/ΔtopS) + topS rescued strain, using a heterologous LdTopIL polyclonal antiserum B) Similar to A but hybridizing to a heterologous LdTopIS polyclonal antiserum C) Similar to A and B but hybridizing to a heterologous β-actin polyclonal antiserum.