High intensity lethal photosensitization of Staph. aureus □ and Strep. pyogenes ▨ with 25 μg ml-1 ICG or P. aeruginosa ■ with 200 μg ml-1 ICG. An equal volume of either 50 μg ml-1 (in the case of Staph. aureus and Strep. pyogenes) or 400 μg ml-1 (in the case of P. aeruginosa) ICG (L-S+ and L+S+) or PBS (L-S- and L+S-) was added to each bacterial suspension, samples were left for 10 minutes in the dark and then irradiated at a fluence rate of 1.37 W cm-2 with a light dose of 411 J cm-2 from a NIR 808 nm laser (L+S- and L+S+) or kept in the dark (L-S+ and L-S-).