Table 2 Primers used in this study
From: Molecular analysis of the emergence of pandemic Vibrio parahaemolyticus
Primer Designation | Primer sequence 5' – 3' | Ta (°C) | Product size (bp) |
|---|---|---|---|
VPaI-2 | Â | Â | Â |
VP0634F | GGGGGAAATAAATGTCTGAAGG | 52.1 | 1363 |
VP0634R | AACACGCCAAGACTCTC | Â | Â |
VP0637F | GGAATAACTCAGAGCTTCG | 52.8 | 1848 |
VP0640R | TAGGCAGTCGTAATTCG | Â | Â |
VP0643F | ATACGCCTGATTGCTTC | 52.0 | 1558 |
VP0643R | TGGTACTATCAACGCCG | Â | Â |
VP0644F | CGTGCTTTTTCTCTTGC | 51.1 | 970 |
VP0644R | CCATATTGCTAGTTAGCTCG | Â | Â |
VPaI-3 | Â | Â | Â |
VP1069F | TAGGGTCGGTGGTGTACTTG | 52.8 | 2041 |
VP1069R | GACTCCACTATTGGTTTA GC | Â | Â |
VP1072F | AGAGTCAGAGGAAAGGGAGG | 50.3 | 2275 |
VP1073R | GTAAATGTTGTGGGTGC | Â | Â |
VP1079F | CTGTCTTCATGCCTTTG | 51.0 | 1691 |
VP1079R | CGCCATTGCTAAACGTC | Â | Â |
VP1083F | CTTACTTATTGGAGGCTGG | 52.1 | 2127 |
VP1083R | GGTGGGTATAAAGGTAACG | Â | Â |
VP1095F | TCTGGTTCGGTATTTGG | 52.7 | 1166 |
VP1096R | CGCAGCATTTCTTGAAG | Â | Â |
VPaI-7 | Â | Â | Â |
VPA1308F | TTAGAACGCATGAGCACCG | 53.1 | 1844 |
VPA1309R | CCACCAAAGTGTTTGTGAG | Â | Â |
VPA1312F | CTACTATCATCACGACGTG | 49.4 | 1487 |
VPA1314R | CGTGCTTATAGCCAGAC | Â | Â |
VPA1317F | GACAGACAGAGATACGCTG | 50.9 | 1366 |
VPA1320R | TTCAGAGGTGTCGCACTTCG | Â | Â |
VPA1321F | CGTGGTGGTTAGTGAATC | 49.1 | 886 |
VPA1321R | AGAGTTGGTTTCGCAGG | Â | Â |
VPA1321F | GACCACTATATTGTTCTCCG | 49.4 | 1480 |
VPA1323R | CTCAGGGATAAATAGGGATG | Â | Â |
VPA1331F | CCAATAATCACCCTCCG | 49.9 | 1857 |
VPA1334R | CTCAGGGATAAATAGGGATG | Â | Â |
VPA1340F | GTCCTTGATTACACCATTGG | 51.8 | 1618 |
VPA1343R | GCACGTAACATCTAAGTTCGTG | Â | Â |
VPA1350F | TGCATCGTCATTTCTCC | 50.4 | 2630 |
VPA1354R | CGTAGATTTCATGGCAG | Â | Â |
VPA1363F | TTTCACTAATGCTGCGG | 51.1 | 2308 |
VPA1365R | GGTCAATATGGCACTATGC | Â | Â |
VPA1380F | TTAGGGGTGTTATGCCG | 48.5 | 816 |
VPA1380R | TTACTGTCTCTGTTGCAGG | Â | Â |
VPA1390F | CCACAACACAAACTGTCC | 50.9 | 2606 |
VPA1393R | AATCCAAGGGGAGTGAC | Â | Â |
VPA1394F | AACGCCGAATTAACCGC | 53.2 | 2356 |
VPA1395R | TCACCCCAATGTACCGTCTG | Â | Â |
VPA1397F | GCGGAGCTGTAATGAAATG | 52.9 | 793 |
VPA1398R | CAACCAACGTATTGTAGCAG | Â | Â |
VPA1400F | ATAGGTCTGTGTAACCCG | 52.3 | 1964 |
VPA1401R | GGTAAAGCTGCGATGAC | Â | Â |
VP0085F | TGCTCGCTGCTATCTAC | 53.0 | 1160 |
VP0085R | CGTTAAATACGCCAGTTGC | Â | Â |
VP0220F | CCCTCAAGTGATTGATCC | 53.0 | 1878 |
VP0220R | AAGATAGCCCCTTGTGG | Â | Â |
VP1399F | CATCTCTTGCTCTTGGAG | 51.0 | 1312 |
VP1400R | TGAGGTCTACAATGAGTCAG | Â | Â |
VP1415F | CGCAATTAAAGGCAGTACG | 51.0 | 2274 |
VP1416R | GACTGAATAAGAGTGCTCG | Â | Â |
VP1556F | TCCCGATTGTAAGTTGC | 53.0 | 1957 |
VP1558R | AGCTAATGCGAATGAGC | Â | Â |
VPA0446F | ACGTTCTTTTGGGATGG | 53.0 | 2376 |
VPA0447R | ACCGAAGCCTTAACACG | Â | Â |
VPA0450F | AATGCGAAAGAAGGCGATAC | 60.0 | 1337 |
VPA0450R | TGCGCTTGTAGATGAGTTGG | Â | Â |
VPA0891F | GTCGCTCTTTATGTTGC | 50.5 | 615 |
VPA0891R | GAAGCCTGTATCAACTGTC | Â | Â |
VPA0894F | TAATGGTCGATGCACTG | 54.0 | 1215 |
VPA0894R | GGATGAGCAAGTCAGTAGC | Â | Â |
VPA0952F | CGAGTGATCCAGTTTTACAC | 52.0 | 2720 |
VPA0953R | AACTACCAGCTAGAAGTGG | Â | Â |
VPA0992F | CCGACATAAAGGGATACTC | 52.0 | 1926 |
VPA0992R | GAAGAAGCACTTGCTCTC | Â | Â |
VPA1443F | CAATCAGCAGCCAGTCGTTA | 60.0 | 1233 |
VPA1443R | CCGGATGTCAAACGGTACTT | Â | Â |
VPA1503F | GTTCGACAATGGCATGTGAG | 60.0 | 801 |
VPA1503R | CGCCAGTATCGACATCACTC | Â | Â |
VPA1655F | CCGTTTTGCTGATGCTACTG | 60.0 | 816 |
VPA1655R | ATGACCACAGTTCCGGAGAG | Â | Â |
VPGyrBF | GTA CTG AAG GGT CTG GAT GC | 54.6 | 742 |
VPGyrBR | ACT GCA TTG CCA CTT CTA CC | Â | Â |
VPmdhF | TGAAAGTAGCCGTTATTGG | 54.0 | 901 |
VPmdhR | CCATTTAGCGTTTCTAGCATTC | Â | Â |
VPGroEL1F | TTTCGGTGCTCCAACCATC | 55.4 | 737 |
VPGroEL1R | GCATTGCTTTACGACGGTC | Â | Â |