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Table 3 Analysis of the specificity of the E. coli A0 34/86 (O83: K24: H31) restriction system

From: Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31)

Plasmid (R-M system)\Phage λ.specificity

λ.0

λ.A

λ.E

λ.C4/1

BAC (none)

1

1

1

1

pFFP30 (EcoAI)

2 Ă— 10-3

1

3 Ă— 10-2

1 Ă— 10-2

pGC1 (EcoEI)

1 Ă— 10-3

4 Ă— 10-2

1

6 Ă— 10-2

C4/1 (EcoA0ORF42P)

2 Ă— 10-4

1 Ă— 10-4

5 Ă— 10-4

1

  1. Individual E. coli DH10B clones harbouring BAC C4/1 with EcoA0ORF42P, plasmid pFFP30(EcoAI) and pGC1(EcoEI) were tested for restriction of λ.vir 0 (phage with no modified sites on its DNA), phage λ.vir specificity of EcoAI, EcoEI and C4/1 were produced on DH10B [pCFF30], DH10B [pGC1] and DH10B [BAC C4/1], respectively. The numbers represent the efficiency of plating (e.o.p.) of lambda phage
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BMC Microbiology

ISSN: 1471-2180

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