Minimum spanning tree based on the diversity of MIRU-VNTR data. Circles show the different clonal complexes identified (maximum neighbour distance: 4 changes; minimum size: 2 MIRU-VNTR types) by the set of 24 loci among the 97 MTBC strains analyzed. The size of each circle is proportional with the number of MIRU-VNTR types belonging to a particular complex. Colours indicate strain previous classification (Table 2). EAI, M. tuberculosis East African Indian; LAM, M. tuberculosis Latin American Mediterranean.