Table 2 Oligonucleotides used in this study
DNA target | Forward Primer [Sequence 5'→3'] | Reverse primer [Sequence 5'→3'] | Annealing T (°C) | Size amplicon (bp) |
|---|---|---|---|---|
Northen Blot (probes) | ||||
rrs | kk4 [TTGGAGAGTTTGATCCTGGCTCAG] | Rac6 [GTGGACTACCAGGGTATCTAATCCT] | 58 | 791 |
sod A | Sod3.1 [ACATCTCGGGTCAGATCAACGAG C] | Sod4 [GACGTTCTTGTACTGCAGGTAG] | 58 | 465 |
kat GII | Cat39 [AAACCCATCCTACTATCG] | Cat40 [GGTTCACGTAGATCAGG] | 50 | 696 |
qRT-PCR | ||||
rrn A-P1 | FoP1 [CTGCTCGTCAGCCTCGAAATCG] | P1R-LC [ACTTCAAAAGATTAGCGCGG] | 55 | 75 |
sod A | Sod12 [CCTTCCAGCTCTACGACCAG] | Sod13 [CCTTCCAGCTCTACGACCAG] | 55 | 114 |
kat GII | katLC1 [GTGGTCCGATCTCATCGTCT] | katLC2 [CAGTAGATGTCCTCCTCGGG] | 55 | 117 |
fur AII | furA-Fw [ACGGCGGACTTCCAGCAG] | furA-Rev [ACGGATGATCGTTTCGGTG] | 62 | 120 |
RPA | ||||
sod A | Sod F8 [ACCTGCACACCCGCAACC] | Sod 3T [PT7-GCTCGTTGATCTGCCCCGA] | 60 | 324 |
kat GII | Cat35 [GACAACCACCACCACGT] | P1.1 T [PT7-TCTCGGCGGGTTCAGTCT] | 50 | 292 |
fur AII | FUR-II-10 [TCGTCTAGCGCCCTCACC] | FUR-II-1T [PT7-ACTCGGGGTCGGGTCACA] | 60 | 177 |
Other primers | ||||
fu rAII-kat GII | furAII-Fo [GACCTACCCGAGGTCTCTCA] | P1.1 [TCTCGGCGGGTTCAGTCT] | 54 | 388 |
pre-rrn A | FoPCL1 [CAAAACAGGGCGGCAAAGC] | cKK4 [GAGCCAGGATCAAACTCTCC] | 58 | 225 |
16S-rrn A | JY15 [CACACTATTGAGTTCTC] | - | - | |