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Table 1 Promoter regions of the sod A, kat GII and fur AII genes of M. fortuitum

From: Transcriptional analysis of Mycobacterium fortuitum cultures upon hydrogen peroxide treatment using the novel standard rrn A-P1

(a)

Gene

P

-35 box

spacing

-10 box

spacing

tsp (+1)

spacing

start codon

sod A

P1

TTGTTC

19

TATACG

4

G

95

GTG

 

P2

TTGCGG

18

AGTGTT

6

G

44

GTG

kat GII

P1

TGCCTG

17

CGGCTT

13

G

90

ATG

 

P2

CACGGC

16

CCGAGG

7

T

76

ATG

fur AII

P1

TCGTCT

20

CGGAAT

12

T

40

GTG

 

P2

TTCTCG

12

TATTCT

5

A

28

GTG

(b)

Mycobacterium species

AT-rich region

spacing

start codon

M. tuberculosis

AGT CTT GAC T AA TTC CAG AAA AG

9

TTG

M. smegmatis

ATT CTT GAC TAA T TC CAG AAA AG

16

GTG

M. fortuitum (fur AII)

ATTCT T GAC TA A TTC CAG AAA AC

16

GTG

  1. (a) Location of the promoters of the sod A, kat GII and fur AII genes of M. fortuitum. Abbreviations: P: promoter; -10 box and -35 box: putative -10 and -35 boxes; tsp (+1): transcription start point.
  2. (b) Alignment of the AT-rich sequences upstream of the fur A genes in mycobacteria. The -35 boxes of M. tuberculosis and M. smegmatis [33] and the -10 box of M. fortuitum are underlined. The tsp+1 nucleotide of the P2 promoter of the fur AII gene of M. fortuitum is bolded.