Figure 4From: Transcriptional analysis of Mycobacterium fortuitum cultures upon hydrogen peroxide treatment using the novel standard rrn A-P1qRT-PCR analysis of kat GII, fur AII and sod A under hydrogen-peroxide treatment. (a) kat GII gene expression; (b) fur AII gene expression; (c) sod A gene expression. The 2-ΔΔCt method was applied to calculate the relative amount of the cDNAs of interest, comparing treated and untreated cultures (see Methods). M. fortuitum cultures were treated with hydrogen-peroxide at the following concentrations: lanes 1 to 3, 0.02 mM; lanes 4 to 6, 0.2 mM; lanes 7 to 9, 2 mM; lanes 10 to 12, 20 mM and lane C, control. Samples of each concentration were treated using increasing length of periods as follows: 30 min (lanes 1, 4, 7 and 10); 60 min (lanes 2, 5, 8 and 11); and 120 min (lanes 3, 6, 9 and 12).Back to article page