Chromosomal organization and similarity of Groups 1 and 2 sigN genes. Panel A schematically shows the location of Group 1 (upper part) and Group 2 (lower part) genes in chromosome 2. Exon coding regions are indicated as boxes and intron sequences as lines. Direction of transcription is indicated by arrowheads. Genomic fragments (contigs) where the genes are located, and the database number (curated and gene prediction) for each gene are shown in the figure. The position of exon 1 of the sigN2 gene was modified with respect to the curated model after re-sequencing of this genomic region. Dashed lines represent the genomic regions eliminated in KO strains; from bp 819699 to 829288 for Group 2, and from bp 2242478 to 2248753 for Group 1. B. Protein sequences deduced for sigN genes were compared trough the use of clustal alignments. Amino acid identity is represented as bars (conservation). Amino acids conserved in all proteins are represented in green. The more divergent regions, used to generate antibodies specific for the proteins of each group are boxed.