Environmental perturbation | Description | Reference (s) |
---|---|---|
Stimulon analysis | Â | Â |
   Temperature shift |  | [8, 9] |
Vegetative growth temperatures | Shift from 26°C to 37°C for 3 hr |  |
Heat shock | Shift from 37°C to 45°C for 10 min |  |
Cold shock | Shift from 37°C to 10°C for 1 hr |  |
   Osmotic stress |  | [10] |
High osmolarity | Treatment with 0.5 M sorbitol for 20 min | Â |
High salinity | Treatment with 0.5 M NaCl for 20 min | Â |
   Oxidative stress | Treatment with 1 mM H2O2 for 30 min at 26°C |  |
   Mild acid stress | Shift from pH7.2 to pH 5.5 for 10 min |  |
   Low Mg2+ | Growth under 10 μM Mg2+ | [15] |
   Iron starvation | Treatment with 100 μM of 2, 2'-dipyridyl for 30 min | [11] |
26°C | 26°C cell cultures |  |
37°C | 37°C cell cultures |  |
   Minimal versus rich medium | TMH versus BHI medium |  |
Exponential phase | Growth to an A620 nm of approximately 0.8 | Â |
Stationary phase | Growth to an A620 nm of approximately 2.0 | Â |
   Growth phase | Exponential versus stationary phase |  |
Minimum medium | Growth in TMH medium | Â |
Rich medium | Growth in BHI medium | Â |
   Antibiotics treatment | 10× MIC concentration of antibiotics for 30 min |  |
Streptomycin | 37°C cell cultures | [12] |
chloramphenicol | 37°C cell cultures |  |
Tetracycline | 37°C cell cultures |  |
Antibacterial peptide | 26°C cell cultures |  |
Regulon analysis | Â | Â |
   PhoP regulon | phoP mutant versus Wide-type (WT) strain |  |
pH5.5 | Growth at pH5.5 | Â |
Low Mg2+ | Growth under 10 μM Mg2+ | [15] |
   Fur regulon | fur mutant versus WT strain | [11] |
26°C | 26°C cell cultures with addition of 40 μM of FeCl3 for 30 min |  |
37°C | 37°C cell cultures with addition of 40 μM of FeCl3 for 30 min |  |
   OxyR regulon | oxyR mutant versus WT strain upon exposure to 1 mM H2O2 for 30 min at 26°C |  |
   OmpR regulon | ompR mutant versus WT strain |  |
High osmolarity | Upon exposure to 0.5 M sorbitol for 20 min | Â |
High salinity | Upon exposure to 0.5 M NaCl for 20 min | Â |