IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates

Table 1 Original source and passage history of C. burnetii isolates used in this study.

Genomic group
RFLP^a	IS1111 PCR^b	Plasmid type	Isolate (Phase)^c	Original source	Passage history^d
I	I	QpH1	9Mi/I/C7 (I)	Montana, tick, 1935	307GP/1TC/1EP
I	I	QpH1	9Mi/II/C4 (II)	Montana, tick, 1935	304GP/90EP/1TC/4EP
I	I	QpH1	9Mi/RSA 514 (II)	Montana, tick, 1935	307GP/1TC/1EP/343 days GP/5EP
I	I	QpH1	Ohio (I)	Ohio, cow's milk, 1956	5EP/2GP/2EP
I	I	QpH1	Australian QD (II)	Australia, human blood, ~1939	177EP
ND^e	I	QpH1	Scottish (ND)	Unknown	Unknown
ND	I	QpH1	Idaho Q (ND)	Unknown	Unknown
ND	I	ND	9Mi/Baca (II)	Montana, tick, 1935	307GP/1TC/1EP/4091 days TC
II	II	QpH1	Henzerling RSA 343 (I)	Italy, human blood, 1945	6GP/25EP/1GP/4EP
II	II	QpH1	Henzerling RSA 331 (II)	Italy, human blood, 1945	6GP/25EP/1GP/36EP
II	II	QpH1	M44 (II)	Greece, human blood, ~1945	1GP/86EP/20GP/50MP/5EP
III	III	QpH1	Q195 (ND)	Idaho, goat	Unknown
IV	IV	QpRS	KAV Q154 (I)	Oregon, human heart valve, 1976	1GP/3EP
IV	IV	QpRS	PAV Q173 (I)	California, human heart valve, 1979	2EP
IV	IV	QpRS	Priscilla Q177 (I)	Montana, goat cotyledon, 1980	Unknown
ND	IV	QpRS	Q238 (ND)	Human heart valve, 1979	3EP
V	V	Plasmidless	Q229 (I)	Human heart valve, 1982	Unknown
ND	V	ND	WAV (ND)	Human heart valve	Unknown
ND	V	ND	Q172 (ND)	Human placenta	3MP/2EP
ND	I	ND	Qiyi (ND)	China, human bone marrow, 1962	Unknown
ND	I	ND	Poker Cat (ND)	Nova Scotia, 1987	Unknown

^aAs determined in previous studies [4, 5].
^bAs determined in this study.
^cPhase of isolate as determined by complement block titration, M.G. Peacock, Rocky Mountain Laboratories, Hamilton, Montana [4].
^dNumbers indicate passage in: GP, guinea pig; TC, tissue culture; EP, embryonated eggs; MP, mice
^eNot determined

ISSN: 1471-2180