DENV-2 tropism for Aedes aegypti salivary glands. Salivary glands were dissected at the time points noted, fixed, and assayed for virus antigen by IFA (n = 30–50). Representative salivary glands (200×) from Chetumal and Rex-D mosquitoes at each time point are shown. DENV-2 virus antigen increased in the salivary glands through out the EIP. Negative controls (A and B) and salivary glands examined with light microscopy (C) are shown. In Rex-D mosquitoes, at 4 dpi viral antigen was typically associated with the fat body surrounding the salivary glands. In contrast salivary gland tissues of Chetumal mosquitoes contained viral antigen at 4 dpi.