Kinetics of DENV-2 midgut infection after oral challenge of Aedes aegypti. Chetumal mosquito midguts dissected at different time points (n = 30 for each time point) were assayed by IFA to detect DENV-2 viral antigen (green). At each time point two representative midguts from Chetumal mosquitoes are presented. Controls are an uninfected midgut and a dengue infected midgut (in red midgut musculature). The magnification was 100×.