Figure 5

Real time PCR of intestinal tissue sections for RRV vp7 gene. Tissue samples were excised from small intestines and total cellular RNA was extracted. Real time PCR was performed for rotavirus vp7 gene. The bars represent geometric mean of the virus load after normalization with housekeeping gapdh gene. The combination of 10 μg HBC and L. rhamnosus GG was able to achieve a statistically significant reduction of virus load (as tested by Fischer's test). *** p < 0.0001, ** p = 0.0005, * p = 0.0016