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Table 4 Antagonism of dopamine-induced growth responsiveness

From: Blockade of catecholamine-induced growth by adrenergic and dopaminergic receptor antagonists in Escherichia coli O157:H7, Salmonella enterica and Yersinia enterocolitica

  Chlorpromazine Concentration (μM)
Species CA 0 0.1 1 10 20 50 75 100 200 300 bN/A
E. coli O157:H7 Dop a 8.00 7.99 7.88 7.82 7.73 7.32 6.75 6.10 5.37 4.98 4.14
  Dop+Fe 8.12          8.16  
  NE 8.30          8.22  
  NE+Fe 8.25          8.31  
  Epi 8.06          7.88  
  Epi+Fe 8.16          8.09  
S. enterica Dop 7.95 7.94 7.88 7.82 7.75 7.67 7.52 7.01 6.17 4.96 4.92
  Dop+Fe 8.16          8.14  
  NE 8.09          7.99  
  NE+Fe 7.99          8.20  
  Epi 8.00          7.88  
  Epi+Fe 8.21          8.16  
Y.enterocolitica Dop 8.00 8.00 8.01 8.00 7.99 7.31 7.07 6.94 5.35 5.04 3.90
  Dop+Fe 8.05          8.03  
  NE 8.05          7.96  
  NE+Fe 8.06          8.02  
  Epi N/D          N/D  
  Epi+Fe N/D          N/D  
  1. E. coli O157:H7, S. enterica and Y. enterocolitica were inoculated at approximately 102 CFU/ml into duplicate 1 mL aliquots of serum-SAPI containing the catecholamines (CA) and concentrations of chlorpromazine shown and incubated and enumerated for growth levels (a, expressed as log-10 CFU/ml)as described in Materials and Methods. NE and Dop were used at 50 μM, and Epi and Fe (Fe(NO3)3) at 100 μM. The results shown are representative data from three separate experiments; data points typically showed variation of less than 3%. Growth levels of non-catecholamine supplemented cultures (b, N/A) are shown for comparison purposes. Chlorpromazine-supplemented cultures showed statistically significant decreases in growth levels (P < 0.001) as compared with control (catecholamine-only supplemented) cultures.
  2. N/D, not determined.