Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia

Table 2 The differentiating characteristics of the thermophilic strain T1^T and its type strains.

Characteristics	1	2	3
Cell width (μm)	0.8–1.0	≥ 0.9	1.5
Cell length (μm)	2.5–6.0	≥ 3	3.5
Spores oval/cylindrical	O/C	O/C	O
Spores position	T	T	T
Oxidase	-	-	+
Growth in:
NaCl 2%	+	+	ND
5%	-	+	ND
Lysozyme broth	-	+	ND
Production of acid from:
L-arabinose	+	-	-
D-xylose	+	+	v
D-mannitol	-	+	+
M-inositol	+	+	-
D-ribose	+	-	+
D-cellobiose	w	+	+
D-galactose	+	v	+
Adonitol	-	+	v
D-lactose	+	-	-
Hydrolysis of:
Gelatin	-	+	-
Casein	-	+	+
Nitrate reduction	+	+	ND
pH range	5.0–9.0	6.0–8.0	6.2–7.5
Temperature (°C)	50–70	37–68	45–70
DNA G+C content (mol %)	52.6	51–55	52–58

Taxa 1, G. zalihae T1^T; 2, G. Kaustophilus (DSM 7263^T); 3, G. thermoleovorans (DSM 5366^T). Characteristics are scored as: +, positive; -, negative; w, weak; v, variable within the group; ND, not determined. Data were obtained from the present study (G. zalihae strain T1^T), Nazina et al. [16], Sunna et al. [3], Kuisiene et al. [14] and Markossian et al. [54] (G. thermoleovorans DSM 5366^T and G. Kaustophilus DSM 7263^T). All strains were negative for anaerobic growth, growth at 30°C, VP reaction, indole production and acid from L-rhamnose, sorbitol. All strains were positive for catalase, hydrolysis of starch, use of citrate and acid from D-fructose and D-glucose.