Skip to main content

Advertisement

Table 2 The differentiating characteristics of the thermophilic strain T1T and its type strains.

From: Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia

Characteristics 1 2 3
Cell width (μm) 0.8–1.0 ≥ 0.9 1.5
Cell length (μm) 2.5–6.0 ≥ 3 3.5
Spores oval/cylindrical O/C O/C O
Spores position T T T
Oxidase - - +
Growth in:    
   NaCl 2% + + ND
   5% - + ND
Lysozyme broth - + ND
Production of acid from:    
   L-arabinose + - -
   D-xylose + + v
   D-mannitol - + +
   M-inositol + + -
   D-ribose + - +
   D-cellobiose w + +
   D-galactose + v +
   Adonitol - + v
   D-lactose + - -
Hydrolysis of:    
   Gelatin - + -
   Casein - + +
Nitrate reduction + + ND
pH range 5.0–9.0 6.0–8.0 6.2–7.5
Temperature (°C) 50–70 37–68 45–70
DNA G+C content (mol %) 52.6 51–55 52–58
  1. Taxa 1, G. zalihae T1T; 2, G. Kaustophilus (DSM 7263T); 3, G. thermoleovorans (DSM 5366T). Characteristics are scored as: +, positive; -, negative; w, weak; v, variable within the group; ND, not determined. Data were obtained from the present study (G. zalihae strain T1T), Nazina et al. [16], Sunna et al. [3], Kuisiene et al. [14] and Markossian et al. [54] (G. thermoleovorans DSM 5366T and G. Kaustophilus DSM 7263T). All strains were negative for anaerobic growth, growth at 30°C, VP reaction, indole production and acid from L-rhamnose, sorbitol. All strains were positive for catalase, hydrolysis of starch, use of citrate and acid from D-fructose and D-glucose.