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Table 2 The differentiating characteristics of the thermophilic strain T1T and its type strains.

From: Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia

Characteristics

1

2

3

Cell width (μm)

0.8–1.0

≥ 0.9

1.5

Cell length (μm)

2.5–6.0

≥ 3

3.5

Spores oval/cylindrical

O/C

O/C

O

Spores position

T

T

T

Oxidase

-

-

+

Growth in:

   

   NaCl 2%

+

+

ND

   5%

-

+

ND

Lysozyme broth

-

+

ND

Production of acid from:

   

   L-arabinose

+

-

-

   D-xylose

+

+

v

   D-mannitol

-

+

+

   M-inositol

+

+

-

   D-ribose

+

-

+

   D-cellobiose

w

+

+

   D-galactose

+

v

+

   Adonitol

-

+

v

   D-lactose

+

-

-

Hydrolysis of:

   

   Gelatin

-

+

-

   Casein

-

+

+

Nitrate reduction

+

+

ND

pH range

5.0–9.0

6.0–8.0

6.2–7.5

Temperature (°C)

50–70

37–68

45–70

DNA G+C content (mol %)

52.6

51–55

52–58

  1. Taxa 1, G. zalihae T1T; 2, G. Kaustophilus (DSM 7263T); 3, G. thermoleovorans (DSM 5366T). Characteristics are scored as: +, positive; -, negative; w, weak; v, variable within the group; ND, not determined. Data were obtained from the present study (G. zalihae strain T1T), Nazina et al. [16], Sunna et al. [3], Kuisiene et al. [14] and Markossian et al. [54] (G. thermoleovorans DSM 5366T and G. Kaustophilus DSM 7263T). All strains were negative for anaerobic growth, growth at 30°C, VP reaction, indole production and acid from L-rhamnose, sorbitol. All strains were positive for catalase, hydrolysis of starch, use of citrate and acid from D-fructose and D-glucose.