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Table 1 Pseudomonas strains, plasmids and oligonucleotides used.

From: The Pseudomonas putida Lon protease is involved in N-acyl homoserine lactone quorum sensing regulation

STRAINS   
P. putida WCS358 Pseudomonas putida, wt [33]
P. putida IBE4 lon1754::Tn5 of WCS358, Kmr This study
P. aeruginosa 18577 PA1803lon437::ISlacz/hah of PAO1, Tcr [34]
PLASMIDS   
pMOSBlue Cloning vector, Ampr Amersham-Pharmacia
pBluescriptKS Cloning vector, Ampr Strategene
pRK2013 Kmr Tra+ Mob+, ColE1 replicon [28]
pET28b Expression vector, kmr Novagen
pBBRmcs5 Broad-host-range vector, Gmr [35]
pSCHIA5 pBluescriptKS carrying a 4.5 Kb BamHI/StuI fragment from IBE4 harboring some WCS358 and a fragment of Tn5, Ampr Kmr This study
pB1A pBluescriptKS carrying a 4.5 Kb EcoRI fragment from WCS358, Ampr This study
pBBRlon pBBRmcs5 carrying a 4.5 Kb EcoRI fragment from WCS358, Genr This study
pPPUR3586H pET28b derivative carrying ppuR of P. putida WCS358 This study
pBBRppuR pBBRmcs5 carrying the ppuR gene amplified from pPuR3586H with primers P0FW and P0RV This study
pPPUI220 pMP220 promoter probe vector carrying the ppuI promoter region, Tcr [14]
pRSA220 pMP220 promoter probe vector carrying the rsaL promoter region, Tcr [14]
OLIGONUCLEOTIDES   
P0FW 5'-GGGTACCAATAATTTTGTTTAACTTTA-3' [12]
P0RV 5'-GGGATCCATTGCTCAGCGGTGGCAGC-3' [12]
FW119 5'-CATGCCATGGCCCTACTGGTAATGG-3' This study
RV122 5'-CCCAAGCTTGGGCGTGATCGATTTTTGC-3' This study
Tn5ext 5'-GAACGTTACCATGTTAGGAGGTC [36]