Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli

Table 4 Frequency of P1 transduction of plsB::kan, plsX::kan, and ygiH::kan mutations into W3110 cells harboring various pairs of plasmids.

Recipient strain									Relative transduction frequency*
	Chromosomal gene		Harboring plasmids						P1 donor strain
Strain	plsX	ygiH	pSTV29	pSTV28 EcplsX	pSTV28 BsplsX	pMW118	pMW118 ygiH	pMW118 yneS	ΔplsB::kan	ΔplsX::kan	ΔygiH::kan
MEC130	+	+	+	-	-	+	-	-	0	56.1	39.4
MEC134	+	+	+	-	-	-	+	-	0	53.7	41.3
MEC136	+	+	+	-	-	-	-	+	0	100	81.7
MEC137	+	+	-	+	-	+	-	-	0	87.8	69.2
MEC139	+	+	-	+	-	-	+	-	0	73.2	100
MEC140	+	+	-	-	+	+	-	-	0	90.2	46.2
MEC142	+	+	-	-	+	-	-	+	100	97.6	90.4

*Transduction frequencies are expressed as percentages of the highest number of Km^r colonies recorded in each experiment, and are depicted as blocks.

ISSN: 1471-2180