Skip to main content

Table 4 Frequency of P1 transduction of plsB::kan, plsX::kan, and ygiH::kan mutations into W3110 cells harboring various pairs of plasmids.

From: Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli

Recipient strain Relative transduction frequency*
  Chromosomal gene Harboring plasmids P1 donor strain
Strain plsX ygiH pSTV29 pSTV28 EcplsX pSTV28 BsplsX pMW118 pMW118 ygiH pMW118 yneS ΔplsB::kan ΔplsX::kan ΔygiH::kan
MEC130 + + + - - + - - 0 56.1 39.4
MEC134 + + + - - - + - 0 53.7 41.3
MEC136 + + + - - - - + 0 100 81.7
MEC137 + + - + - + - - 0 87.8 69.2
MEC139 + + - + - - + - 0 73.2 100
MEC140 + + - - + + - - 0 90.2 46.2
MEC142 + + - - + - - + 100 97.6 90.4
  1. *Transduction frequencies are expressed as percentages of the highest number of Kmr colonies recorded in each experiment, and are depicted as blocks.