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Table 3 Frequency of P1 transduction of plsB::kan, plsX::kan, and ygiH::kan mutations into W3110, strain MEC102, and strain MEC103, harboring pSTV29 -plsB and plsB26

From: Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli

Recipient strain Relative transduction frequency*
  Chromosomal gene Harboring plasmids P1 donor strain
Strain plsX ygiH pSTV29 pSTV29 plsB pSTV29 plsB26 ΔplsB::kan ΔplsX::kan ΔygiH::kan
MEC104 + + + - - 0 66.4 76.9
MEC105 + + - + - 100 100 100
MEC288 + + - - + 61.9 67.7 55.1
MEC110 - + + - - 0 98.2 0
MEC111 - + - + - 100 75 100
MEC289 - + - - + 0 100 0
MEC116 + - + - - 0 0 100
MEC117 + - + - - 100 100 89
MEC290 + - - - + 59.3 0 61.7
  1. *Transduction frequencies are expressed as percentages of the highest number of Kmr colonies recorded in each experiment, and are depicted as blocks.