Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli

Table 3 Frequency of P1 transduction of plsB::kan, plsX::kan, and ygiH::kan mutations into W3110, strain MEC102, and strain MEC103, harboring pSTV29 -plsB and plsB26

Recipient strain						Relative transduction frequency*
	Chromosomal gene		Harboring plasmids			P1 donor strain
Strain	plsX	ygiH	pSTV29	pSTV29 plsB	pSTV29 plsB26	ΔplsB::kan	ΔplsX::kan	ΔygiH::kan
MEC104	+	+	+	-	-	0	66.4	76.9
MEC105	+	+	-	+	-	100	100	100
MEC288	+	+	-	-	+	61.9	67.7	55.1
MEC110	-	+	+	-	-	0	98.2	0
MEC111	-	+	-	+	-	100	75	100
MEC289	-	+	-	-	+	0	100	0
MEC116	+	-	+	-	-	0	0	100
MEC117	+	-	+	-	-	100	100	89
MEC290	+	-	-	-	+	59.3	0	61.7

*Transduction frequencies are expressed as percentages of the highest number of Km^r colonies recorded in each experiment, and are depicted as blocks.

ISSN: 1471-2180