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Table 2 Frequency of P1 transduction of plsB::kan, plsX::kan, and ygiH::kan mutations into strains MEC102 and MEC103 harboring various plasmids

From: Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli

Recipient strain

Relative transduction frequency*

 

Chromosomal gene

Harboring plasmids

P1 donor strain

Strain

plsX

ygiH

pSTV29

pSTV29 plsB

pSTV28 EcplsX

pSTV29 ygiH

pSTV28 BsplsX

pSTV29 yneS

ΔplsB::kan

ΔplsX::kan

ΔygiH::kan

MEC102

-

+

-

-

-

-

-

-

0

100

0

MEC103

+

-

-

-

-

-

-

-

0

0

100

MEC110

-

+

+

-

-

-

-

-

0

55.9

0

MEC111

-

+

-

+

-

-

-

-

100

53.5

59.8

MEC112

-

+

-

-

+

-

-

-

0

56.3

75.3

MEC113

-

+

-

-

-

+

-

-

0

83.1

100

MEC114

-

+

-

-

-

-

+

-

0

45.1

84.2

MEC115

-

+

-

-

-

-

-

+

0

100

92.7

MEC116

+

-

+

-

-

-

-

-

0

0

69.8

MEC117

+

-

-

+

-

-

-

-

100

63.7

85.9

MEC118

+

-

-

-

+

-

-

-

0

99.5

100

MEC119

+

-

-

-

-

+

-

-

0

85.1

73.8

MEC120

+

-

-

-

-

-

+

-

0

36.3

60.5

MEC121

+

-

-

-

-

-

-

+

0

100

96

  1. *Transduction frequencies are expressed as percentages of the highest number of Kmr colonies recorded in each experiment, and are depicted as blocks.