Phospholipid synthesis in B. subtilis yneS-ts , plsC - ts , and IPTG-dependent plsX mutants, under restrictive conditions. Labeling with 32 [Pi] for 5 min, lipid isolation, lipid separation using two-dimensional TLC, and phospholipid detection, are described in Methods. (A) Wild-type cells labeled at 30°C and 42°C. (B) plsC-ts cells (strain MY112) labeled at 30°C and 42°C. (C) yneS-ts cells (strain MY103) labeled at 30°C and 42°C. (D) Pspac-plsX cells (strain MY111) labeled in the presence and absence of IPTG. Abbreviations: 1-acyl-G3P, 1-acylglycerol-phosphate; PA, phosphatidic acid; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; CA, cardiolipin.