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Figure 5 | BMC Microbiology

Figure 5

From: The Yersinia enterocolitica type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1

Figure 5

Differential effect of SycO overproduction on cytosolic Yop levels in the presence of calcium ions. Overproduction of SycO was analyzed in Y. enterocolitica parental strain WA-314 and the isogenic deletion mutants ΔyopO and ΔyopP cultured at 37°C in the presence of 5 mM CaCl2. After 2 h 1 mM IPTG was added and cultivation was continued for another 2 h. Pellet fractions were analyzed by immunoblotting with sera raised aginst YopO, SycO and YopH.

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