Figure 5From: The Yersinia enterocolitica type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1Differential effect of SycO overproduction on cytosolic Yop levels in the presence of calcium ions. Overproduction of SycO was analyzed in Y. enterocolitica parental strain WA-314 and the isogenic deletion mutants ΔyopO and ΔyopP cultured at 37°C in the presence of 5 mM CaCl2. After 2 h 1 mM IPTG was added and cultivation was continued for another 2 h. Pellet fractions were analyzed by immunoblotting with sera raised aginst YopO, SycO and YopH.Back to article page