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Table 3 Properties of the NgoΦ1 CDS' sequences

From: Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae and visualization of productive bacteriophage

NgoΦ1 CDSs

Genome position

Length (aa)

Putative function

Significant matches to proteins in GenBank, scores represent filtered BLASTP, % identities, over stated number of amino acid)

Identity with CDSs

     

NgoΦ2

NgoΦ3

NGO0462

455173–456369

398

phage integrase

VC0847 [Vibrio cholerae] 31, 51% on 406, Int A E. coli K12 32, 51% on 412

  

NGO0463

456725–456994

89

NS*

   

NGO0464

457189–457872

227

hypothetical protein

B3 CDS5 [Bacteriophage B3] 29, 46% on 227

1130, 1131

2e+07

NGO0465

457883–458419

178

NS

   

NGO0466

458530–458745

71

NS

 

1129

1617

NGO0467

458797–459288

163

hypothetical protein

CDS8 [Streptococcus phage Φ-O1205] (pfam SiphoGp 35, 56% on 144, CDS157 [Streptococcus 157 family) Thermophilus phage Sfi21] 31, 51% on 160

1128

1618

NGO0468

459285–459467

60

NS

 

1127

1619

NGO0469

459607–460293

228

replication initiation

BBTVs1gp1 [Banana bunchy top virus] replication protein 26, 43% on 109 primase A2p38 [Lactobacillus casei phage A2] 32, 44% on 87

1126

1620

NGO0470

460362–460523

53

NS

 

1125

1621

NGO0471

460520–460735

71

hypothetical protein

NMB0904 [N. meningitidis MC58] 45, 63% on 61, gp30 [Burkholderia cepacia phage Bcep22] 37, 54% on 48

1124

1622

NGO0472

460949–461236

95

NS

 

1123

1623

NGO0473

461422–461697

91

NS

 

1122

 

NGO0474

461694–462170

158

transcriptional regulator

IrgB [V. cholerae strain N16961 serogroup O1] 26, 62% on 50

1121

 

NGO0475

462203–462403

66

NS

 

1120

1624

NGO0476

462601–463014

137

NS

   

NGO0477

463011–463448

145

transcriptional

EF1886 [Enterococcus faecalis (pfam HTH 3 family) regulator V583] 45, 70% on 44, NMB1204 [N. meningitidis MC58] 32, 59% on 62

  

NGO0478

463489–463926

145

NS

   

NGO0479

464039–464755

238

repressor protein cI

cI [Salmonella typhimurium (pfam peptidase 1630 phage T104] 29, 52% on 222 S24family), HK022 p43 [Enterobacteria phage HK022] 30, 49% on 223 [Pseudomonas phage D3] 26, 42% on 221

 

1630

NGO0480

465140–465295

51

NS

 

1114

1631

NGO0481

465272–465460

62

NS

 

1113

1632

NGO0482

465465–465827

120

NS

   

NGO0483

46633–465860

75

hypothetical protein

Bcep22p22 [B. cepacia phage Bcep22] 32, 52% on 71

1112

1633

NGO0484

465978–467042

354

DNA replication protein

[Phage Aaphi23]33, 45% on 342 Gp54 [Phage HK97] 26, 42% on 123 [Enterobacteria phage P22] 24, 17% on 125

1111

 

NGO0485

467039–468400

453

replicative DNA helicase

(pfam DnaB family, DnaB-C family) [Enterobacteri a phage P1] 34, 53% on 446, CDS74 [Pseudomona s phage D3 ] 32, 55% on 430

1110

 

NGO0486

468417–468701

94

NS

 

1109

 

NGO0487

468740–469234

164

hypothetical protein

SA1787 [Staphylococcus phage phiN315] 41, 51% on 58

1108

1638

NGO0488

469411–469560

49

NS

 

1106

 

NGO0489

469696–470241

181

Endodeoxyribo-nuclease

rus [Phage 82] 34, 47% on102 (pfamRusA family) RUS Sb45 [S. typhimurium phage ST64B ] 31, 44% on 113, rus [Phage HK620] 30, 47% on 96

 

1640

NGO0490

470231–470464

77

hypothetical protein

gp18 [B. cepacia phage Bcep22] 46, 58% on 41

  

NGO0491

470508–470915

135

NS

   

NGO0492

471138–471644

168

NS

   

NGO0493

471965–472186

73

hypothetical protein

SfVp44 [Shigella flexner i phage V] 50, 67% on 34

  

NGO0494

472479–472928

149

terminase small subunit

f6p01 [Enterobacteria phage Sf6] 45, 69% on 122, 3 [Phage HK620] 45, 69% on 122

  

NGO0495

472990–474411

473

terminase large subunit

T1p5324 [Enterobacteria phage T1] (COG 24, 42% on 505, TerL [Phage terminase Aaphi23] 28, 45% on 438,

  

NGO0496

474408–476555

715

phage portal protein

gp34 [Phage phi-C31] (pfam Phage Mu F family) 25, 43% on 254, gp34 [Phage phiB 25], 43% on 258

  

NGO0497

476623–477780

385

protease structural protein

T5 150 [Phage T5] 31, 48% on 137 psiM100p19 [Methanothermobacter wolfeii prophage psiM100], 46% on 140

  

NGO0498

477821–479320

499

hypothetical protein

2 [Equine herpesvirus 1] 23, 30% on 18

  

NGO0499

479327–479689

120

NS

   

NGO0500

479692–480222

176

hypothetical protein

gp8 [Phage phiE125] 30, 44% on 125

  

NGO0501

480370–480702

110

putative virion

SO690 protein, prophage MuSo2, morphogenesis protein [Shewanella oneidensis MR-1] 47, 60% on 53

  

NGO0502

480699–481127

142

NS

   

NGO0503

481153–481926

257

NS

   

NGO0504

481987–482316

109

hypothetical protein

PHG31p11 protein [Aeromonas phage 31] 28, 46% on 88

  

NGO0505

482328–482594

88

NS

   

NGO0506

482594–483193

199

hypothetical protein

gp83 [Phage phi JL001] 25, 46% on 80

  

NGO0507

483190–484044

284

hypothetical protein

gp84 [Phage phi JL001] 42, 58% on 103, D3112p50 [Phage D3112] 32, 47% on 50

  

NGO0508

484046–484477

143

hypothetical protein

BMEI1342 protein) (pfam06940, DUF1287 [Brucella melitenis] 33, 47% on 119

  

NGO0509

484505–484789

93

unknown

CDS114 [Pseudomonas phage phiKZ 43, 60%on 81 transcriptional regulator PP0276 [P. putida KT2440] Cro/cI family 39, 65% on 63

  

NGO0510

485026–489171

1381

hypothetical protein

gp86 [Phage phi JL001] 22,39% on 459 CDS97 [Lactobacillus plantarum phage LP65] 26, 42% on 173 putative phage tail protein HCM2.005c protein [S. enterica] 27, 44% on 180

  

NGO0511

489283–489588

101

NS

   

NGO0512

489593–490528

311

NS

   

NGO0513

489659–490129

156

NS

   

NGO0514

490130–490462

110

NS

   

NGO0515

490591–490824

77

NS

   

NGO0516

490832–491179

115

PemK

   

NGO0517

491179–491415

78

PemI

(pfam SpoVT AbrB family)

  

NGO0518

491622–492161

114

hypothetical protein

NMB0912 protein [N. meningitidis MC58] 32, 45% on 53

 

1649

NGO0519

492162–492506

120

NS

  

1650

NGO0520

492680–492990

103

NS

   

NGO0521

493004–493153

49

hypothetical protein

NMB0989 protein [N. meningitidis MC58] 55, 61% on 47

  

NGO0522

493183–496230

1015

tail length tape

JL00p82 [Phage phi JL001 measure protein 29, 50% on 407 putative tail component [Z6034] protein cryptic prophage CP-933P [E. coli] 27, 47% on 259

  

NGO0523

496290–496769

159

hypothetical protein

NMB0899 [N. meningitidis MC58] 100, 100% on 118

1095

 

NGO0524

497111–498100

329

integrase

[Haemophilus influenzae R2866] (pfam phage integrase family 49, 62% on 200

 

1613

  1. * No significant homology with any gene in the database