Figure 7

Complementation of the λ S am7 mutant with the CDS488 of NgoΦ1. 50 ml of LB medium containing the 1% glucose was inoculated with 1 ml of overnight culture of E. coli MM294 λ111 (cI 857 S am7) carrying pMPMK6::cds448) grown in the LB medium in the presence of 1% glucose at 30°C. After 2.5 h of growth at 30°C the culture was centrifuged at 5000 rpm for 10 min, and the cells suspended in the 50 ml of fresh LB medium without glucose. The λ111 (cI 857 S am7) was induced by incubation of the culture at 44°C for 15 min and the expression of the CDS488 gene was (or not) induced by the addition of arabinose to a final concentration of 0.001%. The white arrow shows the time of CDS488 induction, the black arrow shows the time of heat induction. Symbols: (filled circles), E. coli MM294 λ111 (cI 857 S am7) carrying pMPMK6), no arabinose induction; (open circles), E. coli MM294 λ111 (cI 857 S am7) carrying pMPMK6, with arabinose induction; (open triangles), E. coli MM294 λ111 (cI 857 S am7) carrying pMPMK6::cds488, no induction with arabinose; and (filled triangles), E. coli MM294 λ111 (cI 857 S am7) carrying pMPMK6::cds488, induction with arabinose.