Figure 4
From: Regions important for the adhesin activity of Moraxella catarrhalis Hag
Western blot analysis of Sarkosyl-insoluble OM proteins extracted from E. coli expressing Hag proteins. OM preparations were obtained from E. coli carrying the plasmids pCC1.3 (lane 1), pELO35.Hag (lane 2), pBBHS2.24 (lane 3), pBBHS3.20 (lane 4), pBBHS8.18 (lane 5), pBBHS6.22 (lane 6), pBBHS5.12 (lane 7), pBBHS10.9 (lane 8) and pBBHS10.32 (lane 9). These preparations were resolved by SDS-PAGE, transferred to PVDF membranes and probed with antibodies against the purified recombinant protein His.Hag.CT. The figure is a composite of several western blot experiments in which OM preparations of the negative control (i.e. pCC1.3) and the positive control (i.e. pELO35.Hag) were included. Molecular weight markers are shown to the left in kDa. The numbers at the bottom of the western panel represent the predicted molecular weight of each Hag protein.