Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae

BMC Microbiology

Table 4 Functional categories of phosphoproteins, the variation of protein phosphorylation levels, phosphoprotein orthologs, and the potential association with cytoskeleton-like structure in mycoplasmas

	Proposed function/annotation	MG, MPN number	Stat/Exp growth variation ^a)	Phosphorylated in both M. genitalium and M. pneumoniae ^b)	In Triton-X100 insoluble fraction ^c)
Energy metabolism	Pyruvate dehydrogenase E1-beta chain (PdhB)	MG273 MPN392	↑	Y	+
	Pyruvate dehydrogenase E1-alpha chain (PdhA)	MG274 MPN393	↑	Y	+
	L-Lactate dehydrogenase	MG460 MPN674	-	Y	+
	ATP synthase alpha chain	MG401	-	N	×
	Phosphotransacetylase	MG299	-	N	+
Carbohydrate transport and metabolism genes	Phosphopyruvate hydratase (enolase)	MG407 MPN606	-	Y	×
Translation/transcription	Elongation factor Tu (Tuf)	MG451 MPN665	↑	Y	+
	DNA-directed RNA polymerase delta subunit	MG022 MPN024	↓	Y	×
	Trigger factor (Tig)	MPN331	↑	N	+
	Ribosomal protein S2	MG070 MPN208	-	Y	×
	Transcription elongation factor (GreA)	MG282 MPN401	-	Y	+
Heat shock, chaperones	Heat-shock protein GroEL	MG392 MPN573	↑	Y	+
	Heat-shock protein DnaK	MG305 MPN434	↑	Y	+
	Heat-shock protein DnaJ homolog	MG200	-	N	×
Cytaherance-associated proteins	Cytadherance accessory protein (HMW3)	MG317 MPN452	↓	Y	+
	Protein 65	MG217 MPN309	↑	Y	+
	Putative structural protein involved in cytoskeleton	MG328 MPN474	↑	Y	+
Other functions	Restriction-modification enzyme EcoD	MG438	↓	N	×
	Methionine sulfoxide reductase A	MG408 MPN607	-	Y	×
Unknown functions	Osmotic inducible protein-C-like family.	MG427 MPN625	↑	Y	+
	Hypothetical protein	MG377 MPN555	-	Y	×
	Hypothetical protein H10_orf220L.	MPN295	↑	N	+
	Hypothetical protein	MG342	↑	N	×
	Conserved hypothetical protein	MG218.1	-	N	×

a) The variation of the Pro-Q Diamond dye signals (D)/SYPRO Ruby dye (S) signals between bacteria in stationary phase versus exponential growth phase. "↑" means the phosphorylation level was enhanced in stationary phase, "↓" means the phosphorylation level decreased in stationary phase, and "-"means no significant change was observed.
b) "Y" means the phosphoprotein was detected in both M. genitalium and M. pneumoniae; "N" means the corresponding protein was not identified in both strains.
c) "+" means the detected phosphoprotein was part of a Triton-X100 insoluble fraction putatively associated with the cytoskeleton-like structure as reported in [18], and "x" means it was not.

ISSN: 1471-2180