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Table 3 Identified phosphoproteins of M. pneumoniae

From: Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae

Spot Protein name Gene ID; Protein ID & Locus tag Theoretical pI/Mr Peptide mass fingerprinting
     Match a) peptides Sequence coverage (%) MS & MS/MS score b)
N1 Putative structural protein 877396; S73693 4.94/ 9 (1) 9 99
N2 involved in cytoskeleton (MG328 homolog) (MPN474) 118006.7 22 (2) 24 357
N3 Cytadherence accessory protein HMW3 877094; S73715 (MPN452) 4.67/73676.1 22 (1) 42 883
N4c) P65 876932; S73853 (MPN309) 4.27/47009.1 - - -
N5 Probable DNA-directed RNA 876869; 4.32/ 4 (1) 20 112
N6 polymerase delta subunit RPOE_MYCP 17163.2 3 (0) 15 33
N7   (MPN024)   9 (1) 57 325
N8c) DnaK-type molecular chaperone 876838; S73733 (MPN434) 5.41/65091.4 - - -
N9c) Heat shock protein GroEL 876804; S73595 (MPN573) 5.61/58082.8 - - -
N10 Trigger factor tig 876961; S73831 (MPN331) 6.10/51321.1 15 (1) 37 544
N11 Phosphopyruvate hydratase – (enolase) 876984; S73562 (MPN606) 6.12/49197.4 10 (1) 25 179
N12 Translation elongation factor EF-Tu 877030; S73503 (MPN665) 6.06/43122.3 21 (3) 55 664
N13 Pyruvate dehydrogenase E1-alpha chain 877083; S73771 (MPN393) 6.11/40568.4 19 (0) 63 491
N14 Pyruvate dehydrogenase 877125; S73772 6.47/ 9 (2) 36 221
N15 E1-beta chain (MPN392) 35891.6 15 (1) 52 733
N16 No Significant Hits - - - - -
N17 Hypothetical protein 876832; S73866 7.66/ 9 (1) 34 123
N18 H10_orf220L (MPN295) 25684.2 8 (1) 35 57
N19     9 (2) 33 115
N20     16 (2) 60 244
N21 Ribosomal protein S2 876805; S73949 (MPN208) 9.16/33404.7 20 (1) 48 487
N22 MG427 homolog (Osmotical inducible protein C like family) 877343; S73543 (MPN625) 6.50/15458.9 5 (1) 45 84
N23 c) Transcription elongation factor 877111; S73763 (MPN401) 4.91/18101.5 - - -
N24 c) Methionine sulfoxide reductaseA 876738; S73561 (MPN607) 5.64/18378.6 - - -
N25 c) Hypothetical protein 877212; S73613 (MPN555) 5.58/22433.8 - - -
N26 L-lactate dehydrogenase 877032; S73494 (MPN674) 8.35/33888.1 12 (1) 52 56
  1. a) Number of peptides that match the theoretical digest of the primary protein identified. The number in parentheses is the count of potentially phosphorylated peptides found by Mascot and FindMod (see additional file 1: Putative_Phosphopeptides.pdf, Table B).
  2. b) Combined score for the peptide mass fingerprint including both MS and MS/MS peptide matches for the spectrum. Scores of 95 or greater were considered significant (p < 0.05).
  3. c) Protein spots N4, N8, N9, and N23~ N25 were identified by previous existing proteome maps [6, 14]; "-" means that MS analysis was not performed for these spots. The MS data for N16 was of insufficient quality for identification.