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Table 3 Identified phosphoproteins of M. pneumoniae

From: Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae

Spot

Protein name

Gene ID; Protein ID & Locus tag

Theoretical pI/Mr

Peptide mass fingerprinting

    

Match a) peptides

Sequence coverage (%)

MS & MS/MS score b)

N1

Putative structural protein

877396; S73693

4.94/

9 (1)

9

99

N2

involved in cytoskeleton (MG328 homolog)

(MPN474)

118006.7

22 (2)

24

357

N3

Cytadherence accessory protein HMW3

877094; S73715 (MPN452)

4.67/73676.1

22 (1)

42

883

N4c)

P65

876932; S73853 (MPN309)

4.27/47009.1

-

-

-

N5

Probable DNA-directed RNA

876869;

4.32/

4 (1)

20

112

N6

polymerase delta subunit

RPOE_MYCP

17163.2

3 (0)

15

33

N7

 

(MPN024)

 

9 (1)

57

325

N8c)

DnaK-type molecular chaperone

876838; S73733 (MPN434)

5.41/65091.4

-

-

-

N9c)

Heat shock protein GroEL

876804; S73595 (MPN573)

5.61/58082.8

-

-

-

N10

Trigger factor tig

876961; S73831 (MPN331)

6.10/51321.1

15 (1)

37

544

N11

Phosphopyruvate hydratase – (enolase)

876984; S73562 (MPN606)

6.12/49197.4

10 (1)

25

179

N12

Translation elongation factor EF-Tu

877030; S73503 (MPN665)

6.06/43122.3

21 (3)

55

664

N13

Pyruvate dehydrogenase E1-alpha chain

877083; S73771 (MPN393)

6.11/40568.4

19 (0)

63

491

N14

Pyruvate dehydrogenase

877125; S73772

6.47/

9 (2)

36

221

N15

E1-beta chain

(MPN392)

35891.6

15 (1)

52

733

N16

No Significant Hits

-

-

-

-

-

N17

Hypothetical protein

876832; S73866

7.66/

9 (1)

34

123

N18

H10_orf220L

(MPN295)

25684.2

8 (1)

35

57

N19

   

9 (2)

33

115

N20

   

16 (2)

60

244

N21

Ribosomal protein S2

876805; S73949 (MPN208)

9.16/33404.7

20 (1)

48

487

N22

MG427 homolog (Osmotical inducible protein C like family)

877343; S73543 (MPN625)

6.50/15458.9

5 (1)

45

84

N23 c)

Transcription elongation factor

877111; S73763 (MPN401)

4.91/18101.5

-

-

-

N24 c)

Methionine sulfoxide reductaseA

876738; S73561 (MPN607)

5.64/18378.6

-

-

-

N25 c)

Hypothetical protein

877212; S73613 (MPN555)

5.58/22433.8

-

-

-

N26

L-lactate dehydrogenase

877032; S73494 (MPN674)

8.35/33888.1

12 (1)

52

56

  1. a) Number of peptides that match the theoretical digest of the primary protein identified. The number in parentheses is the count of potentially phosphorylated peptides found by Mascot and FindMod (see additional file 1: Putative_Phosphopeptides.pdf, Table B).
  2. b) Combined score for the peptide mass fingerprint including both MS and MS/MS peptide matches for the spectrum. Scores of 95 or greater were considered significant (p < 0.05).
  3. c) Protein spots N4, N8, N9, and N23~ N25 were identified by previous existing proteome maps [6, 14]; "-" means that MS analysis was not performed for these spots. The MS data for N16 was of insufficient quality for identification.
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BMC Microbiology

ISSN: 1471-2180

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