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Table 2 Identified phosphoproteins of M. genitalium

From: Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae

Spot Protein name Gene ID; Protein ID & Locus tag Theoretical pI/M r Peptide mass fingerprinting
     Match peptides a) Sequence coverage coverage (%) MS & MS/MS score b)
G1 Putative structural protein involved in cytoskeleton 875488; C64236 (MG328) 4.61/88353.9 26 (1) 39 811
G2 Heat shock protein dna J homolog MG200 875694; A64222 (MG200) 4.86/68494.8 17 (2) 30 396
G3 P65 protein 875361; I64223 (MG217) 4.59/44636.8 17 (1) 33 464
G4 c) Conserved hypothetical protein 875359; AAC71439 (MG218.1) 4.96/39701.4 17(3) 51 461
G5 DNA-directed RNA polymerase delta chain 875707; D64202 (MG022) 4.48/17046.3 6 (0) 55 204
G6 Transcription elongation factor gre A homolog 875338; B64231 (MG282) 4.99/18151.5 13 (1) 81 857
G7 Heat shock protein GroEL 875662; D64243 (MG392) 5.82/58318.4 37 (2) 67 1030
G8 DnaK-type molecular chaperone 875435; G64233; (MG305) 6.79/65010.5 28 (0) 46 997
G9     29 (1) 44 1120
G10     26 (2) 45 1160
G11 Cytadherence-accessory protein 875571; A64235 7.01/ 22 (2) 40 401
G12 (hmw3) homolog (MG317) 68679.1 24 (2) 42 515
G13 Pyruvate dehydrogenase E1-alpha chain 875404; C64230 (MG274) 5.55/40625.3 13 (1) 45 651
G14 Phosphopyruvate hydratase -(enolase) 875644; A64245 (MG407) 6.63/49987.9 15 (2) 45 297
G15 Translation elongation factor EF-Tu 875605; EFYMTG (MG451) 6.18/42963.3 14 (2) 44 582
G16 Restriction-modification enzyme 875615; D64248 7.58/ 26 (6) 52 770
G17 EcoD specificity chain (MG438) 44082.8 23 (6) 46 409
G18 Pyruvate dehydrogenase E1-beta 875399; B64230 6.04/ 13 (1) 41 697
G19 chain (MG273) 36002.8 13 (1) 41 749
G20 Phosphate acetyltransferase 875564; A64233 (MG299) 6.97/35446.5 13 (0) 47 575
G21 c) Hypothetical protein 875676; G64241 (MG377 6.24/22635 27(5) 74 864
G22 c)     20(3) 66 642
G23 Methionine sulfoxide reductase A 875645; B64245 (MG408) 6.18/18403.2 6 (1) 33 147
G24 Conserved hypothetical protein 875626; B64247 (MG427) 6.19/ 7(1) 82 220
G25    15593 4(0) 30  
G26     7(1) 82  
G27 Hypothetical protein MG342 875477; H64237 (MG342) 8.37/ 8 (1) 56 248
G28    19115.9 4 (1) 30 82
G29 c) ATP synthase alpha chain 875650; (MG401) 6.70/57215.4 22(6) 55 220
G30 c) L-lactate dehydrogenase and 875596; (MG460) 8.76/33966.2 28(1) 72 566 d)
  Ribosomal protein S2 875215 (MG070) 9.36/32746.8 17(4) 46  
G31 c) L-lactate dehydrogenase 875596; (MG460) 8.76/33966.2 25(3) 70 396
  1. a) Number of peptides that match the theoretical digest of the primary protein identified. The number in parentheses is the count of potentially phosphorylated peptides found by Mascot and FindMod (see additional file 1: Putative_Phosphopeptides.pdf, Table A).
  2. b) Combined score for the peptide mass fingerprint including both MS and MS/MS peptide matches for the spectrum. Scores of 95 or greater were considered significant (p < 0.05).
  3. c) Identified proteins detected by labeling with 33P-phosphoric acid.
  4. d) Spot G30 was identified to contain both L-lactate dehydrogenase and Ribosomal protein S2. The MS & MS/MS score shown here belongs to the L-lactate dehydrogenase.