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Table 2 Identified phosphoproteins of M. genitalium

From: Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae

Spot

Protein name

Gene ID; Protein ID & Locus tag

Theoretical pI/M r

Peptide mass fingerprinting

    

Match peptides a)

Sequence coverage coverage (%)

MS & MS/MS score b)

G1

Putative structural protein involved in cytoskeleton

875488; C64236 (MG328)

4.61/88353.9

26 (1)

39

811

G2

Heat shock protein dna J homolog MG200

875694; A64222 (MG200)

4.86/68494.8

17 (2)

30

396

G3

P65 protein

875361; I64223 (MG217)

4.59/44636.8

17 (1)

33

464

G4 c)

Conserved hypothetical protein

875359; AAC71439 (MG218.1)

4.96/39701.4

17(3)

51

461

G5

DNA-directed RNA polymerase delta chain

875707; D64202 (MG022)

4.48/17046.3

6 (0)

55

204

G6

Transcription elongation factor gre A homolog

875338; B64231 (MG282)

4.99/18151.5

13 (1)

81

857

G7

Heat shock protein GroEL

875662; D64243 (MG392)

5.82/58318.4

37 (2)

67

1030

G8

DnaK-type molecular chaperone

875435; G64233; (MG305)

6.79/65010.5

28 (0)

46

997

G9

   

29 (1)

44

1120

G10

   

26 (2)

45

1160

G11

Cytadherence-accessory protein

875571; A64235

7.01/

22 (2)

40

401

G12

(hmw3) homolog

(MG317)

68679.1

24 (2)

42

515

G13

Pyruvate dehydrogenase E1-alpha chain

875404; C64230 (MG274)

5.55/40625.3

13 (1)

45

651

G14

Phosphopyruvate hydratase -(enolase)

875644; A64245 (MG407)

6.63/49987.9

15 (2)

45

297

G15

Translation elongation factor EF-Tu

875605; EFYMTG (MG451)

6.18/42963.3

14 (2)

44

582

G16

Restriction-modification enzyme

875615; D64248

7.58/

26 (6)

52

770

G17

EcoD specificity chain

(MG438)

44082.8

23 (6)

46

409

G18

Pyruvate dehydrogenase E1-beta

875399; B64230

6.04/

13 (1)

41

697

G19

chain

(MG273)

36002.8

13 (1)

41

749

G20

Phosphate acetyltransferase

875564; A64233 (MG299)

6.97/35446.5

13 (0)

47

575

G21 c)

Hypothetical protein

875676; G64241 (MG377

6.24/22635

27(5)

74

864

G22 c)

   

20(3)

66

642

G23

Methionine sulfoxide reductase A

875645; B64245 (MG408)

6.18/18403.2

6 (1)

33

147

G24

Conserved hypothetical protein

875626; B64247 (MG427)

6.19/

7(1)

82

220

G25

  

15593

4(0)

30

 

G26

   

7(1)

82

 

G27

Hypothetical protein MG342

875477; H64237 (MG342)

8.37/

8 (1)

56

248

G28

  

19115.9

4 (1)

30

82

G29 c)

ATP synthase alpha chain

875650; (MG401)

6.70/57215.4

22(6)

55

220

G30 c)

L-lactate dehydrogenase and

875596; (MG460)

8.76/33966.2

28(1)

72

566 d)

 

Ribosomal protein S2

875215 (MG070)

9.36/32746.8

17(4)

46

 

G31 c)

L-lactate dehydrogenase

875596; (MG460)

8.76/33966.2

25(3)

70

396

  1. a) Number of peptides that match the theoretical digest of the primary protein identified. The number in parentheses is the count of potentially phosphorylated peptides found by Mascot and FindMod (see additional file 1: Putative_Phosphopeptides.pdf, Table A).
  2. b) Combined score for the peptide mass fingerprint including both MS and MS/MS peptide matches for the spectrum. Scores of 95 or greater were considered significant (p < 0.05).
  3. c) Identified proteins detected by labeling with 33P-phosphoric acid.
  4. d) Spot G30 was identified to contain both L-lactate dehydrogenase and Ribosomal protein S2. The MS & MS/MS score shown here belongs to the L-lactate dehydrogenase.
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BMC Microbiology

ISSN: 1471-2180

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