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Table 2 Strains and plasmids

From: Variability in SCC mecN1 spreading among injection drug users in Zurich, Switzerland

Strain Relevant genotype Phenotype Origin, Reference
S. aureus
   CHE482 CC45, ST45, SCCmecN1 (dfrA, fusB1), blaZ Mcr, Tmr, Far, SxR IMM collection, University Zurich
   ME21 CHE482ΔSCCmecN1, blaZ Mcs, Tms, Fas, SxR this study
   CHE482Δ CHE482Δ'SCCmecN1 (fusB1), blaZ Mcs, Tms, Far, SxR this study
   ZH81 SCCmecN1 (dfrA, fusB1), blaZ Mcr, Tmr, Far [9]
   ME141 ZH81ΔSCCmecN1, blaZ Mcs, Tms, Fas this study
   ZH4 SCCmecN1(dfrA, fusB1), blaZ Mcr, Tmr, Cpr, Far, SxR [9]
   ME135 ZH4ΔSCCmecN1, blaZ Mcs, Tms, Cpr, Fas, SxR this study
   ZH43 SCCmecN1 (dfrA, fusB1), blaZ, fusA Mcr, Tmr, Cpr, Far [9]
   ME138 ZH43ΔSCCmecN1, blaZ, fusA Mcs, Tms, Cpr Far this study
   HDE288 Pediatric clone, type 4 ccr complex Mcr [24, 43]
E. coli
   DH5α restriction-negative strain for cloning   Invitrogen
Plasmids
   pYT3 ori(ts), S. aureus, tetL Tcr [35]
   pSR3-1 ori(ts) S. aureus, ccrAB2 genes, tetL Tcr [35]
   pAW17 S. aureus-E. coli shuttle vector, aac-aph Kmr [44]
   pME15 pAW17 ccrAB4-1CHE482 Kmr This study
   pME21 pYT3 ccrAB4-1CHE482 Tcr This study
   pME22 pYT3 ccrAB4-2CHE482 Tcr This study
  1. Abbreviations: Ap, ampicillin; CC, clonal complex; Cp, ciprofloxacin; Fa, fusidic acid; Gm, gentamicin; Km, kanamycin; Mc, methicillin; Ox, oxacillin; ST, sequence type; Sx, sulfomethoxazole; Tc, tetracycline; Tm, trimethoprim; ts, temperature sensitive.