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Table 2 Cytoplasmic protein pattern of wild type strain ATCC13032 and ftsH deletion mutant. The listed proteins differ in their abundance of a factor of at least two.

From: A proteomic study of Corynebacterium glutamicumAAA+ protease FtsH

Spot #

NCgl #

Protein (Gene)

ΔftsH/wild type ratio

MW kDa

pI

1

0670

biotin carboxylase/biotin carboxyl carrier protein (accBC)

53.48

63.5

5.02

2

1526

glyceraldehyde-3-phosphate dehydrogenase (gap)

4.24

36.2

5.16

3

2247

malate synthase (aceB)

2.95

82.5

5.0

4

2248

isocitrate lyase (aceA)

2.65

47.2

4.92

5

1985

conserved hypothetical protein

2.31

122.8

4.85

6

1094

homocysteine methyltransferase (metE)

2.16

81.3

4.78

7

2037

maltooligosyl trehalose synthase (treY)

0.48

90.5

5.03

8

1177

1,4-alpha-glucan branching enzyme (glgB)

0.48

82.6

4.99

9

1023

putative nicotinate-nucleotide pyrophosphorylase

0.47

29.4

5.22

10

2431

nicotinic acid phosphoribosyltransferase

0.47

48.0

5.22

11

0187

L-gulonolactone oxidase

0.47

53.0

5.68

12

0578

inositol-monophosphate dehydrogenase (guaB2)

0.47

53.4

5.99

13

0094

AMP nucleosidase (amn)

0.46

53.7

5.23

14

0358

transcriptional regulator, MerR family (ramB)

0.46

53.9

6.29

15

0704

putative DNA helicase

0.46

84.1

5.35

16

2718

sulfite reductase (hemoprotein) (cysI)

0.43

63.0

5.53

17

0251

catalase (katA)

0.42

58.7

5.18

18

0200

quinone oxidoreductase

0.41

33.2

4.99

19

2133

glutamine synthetase (glnA)

0.41

53.3

4.90

20

0471

DNA-directed RNA polymerase beta chain (rpoB)

0.41

128.8

4.86

21

1446

aspartate ammonia-lyase (aspartase) (aspA)

0.4

57.6

5.69

22

1440

ATPases of the AAA+ class

0.4

58

4.91

23

1835

polyphosphate glucokinase (ppgK)

0.4

26.7

4.97

24

0371

probable formyltetrahydrofolate deformylase protein (purU)

0.39

34.3

5.68

25

2986

N-acetymuramyl-L-alanine amidase (cwlM)

0.38

44.5

5.63

26

0967

fumarate hydratase (fum)

0.37

49.8

5.06

27

1442

aspartyl aminopeptidase (pepC)

0.36

44.9

5.10

28

2817

putative L-lactate dehydrogenase (lldA)

0.34

45.7

5.72

29

2126

dihydrolipoamide succinyltransferase (sucB)

0.34

70.9

4.26

30

1526

glyceraldehyde-3-phosphate dehydrogenase (gap)

0.34

36.0

5.16

31

1523

phosphoenolpyruvate carboxylase (ppc)

0.33

103.2

4.92

32

0251

catalase (katA)

0.29

58.7

5.18

33

1064

succinyl-diaminopimelate desuccinylase (dapE)

0.29

40.0

4.84

34

2586

inositol-monophosphate dehydrogenase (guaB1)

0.28

50.8

6.39

35

2487

GCN5-related N-acetyltransferase

0.27

32.1

5.86

36

2167

pyruvate dehydrogenase E1 component (aceE)

0.27

102.8

5.26

37

1151

acyl-CoA synthetase (fadD4)

0.26

63.7

5.08

38

0360

succinate dehydrogenase A (sdhA)

0.25

74.7

5.37

39

0570

predicted carbohydrate kinase

0.19

60.0

5.08

40

0707

superfamily II DNA/RNA helicase, SNF2 family

0.16

106.9

5.65

41

1219

dihydroxy-acid dehydratase (ilvD)

0.16

64.2

5.18

42

1513

transaldolase (tal)

0.15

38.3

4.47

43

2602

GTP cyclohydrolase (folE)

0.08

22.0

6.08