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Table 4 Description of the plasmids and bacterial strains used in this study

From: Brucella suis urease encoded by ure 1 but not ure 2 is necessary for intestinal infection of BALB/c mice

Plasmid or strain Description Source or reference
Plasmids   
pCR2.1 TA cloning vector, 3.9-kb, Ampr Invitrogen
pCRure 1 pCR2.1 with 2.2-kb insert containing the B. suis ure 1D, ure 1A, ure 1B, ure 1C genes; Ampr This study
PCRure2 ABC pCR2.1 with 2.2-kb insert containing the B. suis ure 2A, ure 2B, ure 2C genes; Ampr This study
pGEM-3Z Cloning vector, 2.74-kb, Ampr Promega
pGEMure 1 pGEM-3Z with 2.2-kb insert containing the B. suis ure 1D, ure 1A, ure 1B, and ure 1C genes; Ampr This study
pGEMure2 ABC pGEM-3Z with 2.2-kb insert containing the B. suis ure 2A, ure 2B, and ure 2C genes; Ampr This study
pGEMure2 ABC-2 pGEM-3Z with 2.9-kb insert containing the B. suis ure 2A, ure 2B, ure 2C genes; Ampr This study
pUC4K Cloning vector, 3.9-kb, Kanr, Ampr Pharmacia
pGEMure1 K pGEMure 1 with 0.9-kb Nco I fragment deleted and blunt ended and a 1.6-kb Pvu II-cut and blunt-ended Kanr cassette from pUC4K ligated, Kanr, Ampr This study
pGEMure2 ABCK pGEMure2 with 0.6-kb Sac II fragment deleted and blunt ended and a 1.6-kb Pvu II-cut and blunt-ended Kanr cassette from pUC4K ligated, Kanr, Ampr This study
pGEMure2 ABCC pGEMure2 with 1.2-kb Cla I and Mfe I fragment deleted and blunt ended and a 1.7-kb Eco52 I plus Kpn I-cut and blunt-ended Cmr cassette from pBBR4MCS ligated, Cmr, Ampr This study
pBBR4MCS Broad-host-range vector; Cmr [27]
Strains   
Escherichia coli Top10   
  F- mcrA Δ(mrr-hsd RMS-mcr BC) Φ80lac ZΔM15 Δlac X74 deo Rrec A1 ara D139 Δ(ara-leu) 7697 gal U gal K rps L (StrR) end A1 nup G Invitrogen
Brucella suis   
1330 Parent-type, smooth strain G.G. Schurig
1330Δure1 K ure1 DA deleted mutant of 1330, Kanr This study
1330Δure2 K ure2 BC deleted mutant of 1330, Kanr This study
1330Δure2 C ure2 ABC deleted mutant of 1330, Cmr This study
1330Δure1ure2C ure1 DA and ure2 ABC deleted mutant of 1330, Cm r, Kanr This study