Skip to main content

Table 4 Description of the plasmids and bacterial strains used in this study

From: Brucella suis urease encoded by ure 1 but not ure 2 is necessary for intestinal infection of BALB/c mice

Plasmid or strain

Description

Source or reference

Plasmids

  

pCR2.1

TA cloning vector, 3.9-kb, Ampr

Invitrogen

pCRure 1

pCR2.1 with 2.2-kb insert containing the B. suis ure 1D, ure 1A, ure 1B, ure 1C genes; Ampr

This study

PCRure2 ABC

pCR2.1 with 2.2-kb insert containing the B. suis ure 2A, ure 2B, ure 2C genes; Ampr

This study

pGEM-3Z

Cloning vector, 2.74-kb, Ampr

Promega

pGEMure 1

pGEM-3Z with 2.2-kb insert containing the B. suis ure 1D, ure 1A, ure 1B, and ure 1C genes; Ampr

This study

pGEMure2 ABC

pGEM-3Z with 2.2-kb insert containing the B. suis ure 2A, ure 2B, and ure 2C genes; Ampr

This study

pGEMure2 ABC-2

pGEM-3Z with 2.9-kb insert containing the B. suis ure 2A, ure 2B, ure 2C genes; Ampr

This study

pUC4K

Cloning vector, 3.9-kb, Kanr, Ampr

Pharmacia

pGEMure1 K

pGEMure 1 with 0.9-kb Nco I fragment deleted and blunt ended and a 1.6-kb Pvu II-cut and blunt-ended Kanr cassette from pUC4K ligated, Kanr, Ampr

This study

pGEMure2 ABCK

pGEMure2 with 0.6-kb Sac II fragment deleted and blunt ended and a 1.6-kb Pvu II-cut and blunt-ended Kanr cassette from pUC4K ligated, Kanr, Ampr

This study

pGEMure2 ABCC

pGEMure2 with 1.2-kb Cla I and Mfe I fragment deleted and blunt ended and a 1.7-kb Eco52 I plus Kpn I-cut and blunt-ended Cmr cassette from pBBR4MCS ligated, Cmr, Ampr

This study

pBBR4MCS

Broad-host-range vector; Cmr

[27]

Strains

  

Escherichia coli Top10

  
 

F- mcrA Δ(mrr-hsd RMS-mcr BC) Φ80lac ZΔM15 Δlac X74 deo Rrec A1 ara D139 Δ(ara-leu) 7697 gal U gal K rps L (StrR) end A1 nup G

Invitrogen

Brucella suis

  

1330

Parent-type, smooth strain

G.G. Schurig

1330Δure1 K

ure1 DA deleted mutant of 1330, Kanr

This study

1330Δure2 K

ure2 BC deleted mutant of 1330, Kanr

This study

1330Δure2 C

ure2 ABC deleted mutant of 1330, Cmr

This study

1330Δure1 KΔure2C

ure1 DA and ure2 ABC deleted mutant of 1330, Cm r, Kanr

This study