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Table 2 B. suis strains: generation time (doubling time, h) in TSB and urease activity in urease test broth.

From: Brucella suis urease encoded by ure 1 but not ure 2 is necessary for intestinal infection of BALB/c mice

Strain

Doubling time (h)*

Urease activity

  

Qualitative**

Quantitative***

1330

5.1

+

9.28

1330Δure 1K

5.8

-

~0

1330Δure 2K

5.3

+

9.38

1330Δure 2C

5.1

+

8.28

1330Δure 1K1330Δure 2C

6.5

-

~0

  1. *A representative experiment was used to calculate the generation time.
  2. ** + represents positive or pink color and – represents negative or yellow color of the uninoculated test broth; the color remained unchanged even 96 h after inoculation
  3. ***Specific activity μmoles/min/mg of protein.