Table 2 Primers used
From: Characterization of a small PlcR-regulated gene co-expressed with cereolysin O
Primer name | 5'-3' sequence* | Restriction sites |
|---|---|---|
Ppep1-L | GATACTGCAG CCTTATGGGCCAATAGCAGT | Pst I |
Ppep1-R | CGTCGGATCC TGATTGATAAATGATTGCTAACTAA | Bam HI |
Ppep2-L | CGCAAGCTT TCTAAACAAGGAATCCTACAAAG | Hin dIII |
Ppep2-R | CGCGGATCC CTCCTCTTTTTCGTATTAAGATG | Bam HI |
Ppep3-L | CGCAAGCTT GGAAATAGTGGGTCTAGAACAT | Hin dIII |
Ppep3-R | CGCGGATCC CCTCTTTTGTTAATACTGGGA | Bam HI |
Extsnclo | CTAACTAATAAACATGCAAGGAAC | Â |