From: Characterization of a small PlcR-regulated gene co-expressed with cereolysin O
Strain or plasmid | Relevant genotype | Source or reference |
---|---|---|
Strains | Â | Â |
B. cereus ATCC14579 | Â | laboratory collection |
B. cereus Δ plcR | ATCC14579 plcR::Km | [5] |
B. subtilis 168 | Â | laboratory collection |
B. cereus F4430/73 | Â | laboratory collection |
B. thuringiensis 407 Cry- | Â | laboratory collection |
E. coli ET12567 | (F- dam-13::Tn9 dcm-6 hsdM hsdR recF143 zjj-202::Tn10 galK2 galT22 ara14 pacY1 xyl-5 leuB6 thi-1) | laboratory collection |
Proteus mirabilis | Â | N. Boemare |
Pseudomonas aeruginosa | Â | N. Boemare |
Salmonella spp. | Â | N. Boemare |
Enterococcus faecalis | Â | P. Serror |
Listeria innocua | Â | laboratory collection |
Streptococcus agalactiae | Â | P. Serror |
Staphylococcus aureus | Â | P. Serror |
Plasmids | Â | Â |
pHT304-18'Z | ApR and EmR cloning vehicle; lacZ reporter gene | [38] |
pHT-Ppep1'Z | 433 bp region upstream from clo start codon inserted between Pst I and Bam HI sites of pHT304-18'Z | this work |
pHT-Ppep2'Z | 448 bp region upstream from pep2 start codon inserted between Hin dIII and Bam HI sites of pHT304-18'Z | this work |
pHT-Ppep3'Z | 480 bp region upstream from pep3 start codon inserted between Hin dIII and Bam HI sites of pHT304-18'Z | this work |