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Table 1 Strains and plasmids used in this study

From: Characterization of a small PlcR-regulated gene co-expressed with cereolysin O

Strain or plasmid Relevant genotype Source or reference
Strains   
B. cereus ATCC14579   laboratory collection
B. cereus Δ plcR ATCC14579 plcR::Km [5]
B. subtilis 168   laboratory collection
B. cereus F4430/73   laboratory collection
B. thuringiensis 407 Cry-   laboratory collection
E. coli ET12567 (F- dam-13::Tn9 dcm-6 hsdM hsdR recF143 zjj-202::Tn10 galK2 galT22 ara14 pacY1 xyl-5 leuB6 thi-1) laboratory collection
Proteus mirabilis   N. Boemare
Pseudomonas aeruginosa   N. Boemare
Salmonella spp.   N. Boemare
Enterococcus faecalis   P. Serror
Listeria innocua   laboratory collection
Streptococcus agalactiae   P. Serror
Staphylococcus aureus   P. Serror
Plasmids   
pHT304-18'Z ApR and EmR cloning vehicle; lacZ reporter gene [38]
pHT-Ppep1'Z 433 bp region upstream from clo start codon inserted between Pst I and Bam HI sites of pHT304-18'Z this work
pHT-Ppep2'Z 448 bp region upstream from pep2 start codon inserted between Hin dIII and Bam HI sites of pHT304-18'Z this work
pHT-Ppep3'Z 480 bp region upstream from pep3 start codon inserted between Hin dIII and Bam HI sites of pHT304-18'Z this work
  1. Km, kanamycin; Ap, ampicillin; Em, erythromycin.