Alignment of Pep sequences identified in members of the B. cereus group. Diverging amino acids are shown in grey boxes. Alignments were performed with the Multalin version 5.4.1 program . The numbers indicate copy number (1, 2 or 3) in the available genome sequences from the B. cereus group. Each number 1 corresponds to a Pep orthologue encoded by an ORF positioned upstream from a cholesterol-dependent cytolysin-encoding gene. Bc14579: B. cereus ATCC14579; Bc10987: B. cereus ATCC10987; BcZK: B. cereus EL33; BcG9241: B. cereus G9241; Btk: B. thuringiensis serovar konkukian strain 97-27; Bt407: B.thuringiensis strain 407Cry-; Bti: B. thuringiensis serovar israelensis ATCC 35646. For B. anthracis, the finished and unfinished genome sequences of the various strains gave the same Pep sequences, which are indicated only once. The B. anthracis strains tested were: strain Ames Ancestor, strain Ames, strain Sterne, strain Kruger B, strain A1055, strain CNEVA-9066, strain Western North America USA6153, strain Vollum, and strain Australia 94. In the B. anthracis strain A2012 unfinished genome sequence, only orthologue number 1 was identified. *: Pep orthologue identified in an unfinished genome sequence.