Figure 1
From: Characterization of a small PlcR-regulated gene co-expressed with cereolysin O
Map of the B. cereus ATCC 14579 pep-clo locus. (A) The PlcR recognition site (bold underlined), -10 and -35 boxes (underlined) and the putative RBS binding site (bold italic) are indicated. The transcription initiation site (+1) is indicated by the arrow. (B) Total RNA (20 μg) extracted from B. cereus at the onset of stationary phase (T0), two hours (T2) and four hours (T4) after T0 was subjected to primer extension analysis, using an oligonucleotide binding 65 nucleotides downstream from the Clo start codon. The same oligonucleotide was used to prime dideoxy sequencing reactions from the corresponding region obtained by PCR amplification (lanes C, T, A, G).