Table 4 Primer sequences used for nested multiplex PCR
Genus specific primers (First PCR) | |
|---|---|
Entamoeba genus | E-1 5' TAAGATGCACGAGAGCGAAA 3' (forward primer) E-2 5' GTACAAAGGGCAGGGACGTA 3' (reverse primer) |
Species specific primers (Second nested multiplex PCR) | |
E. histolytica species | EH-1 5' AAGCATTGTTTCTAGATCTGAG 3' (forward primer) EH-2 5' AAGAGGTCTAACCGAAATTAG 3' (reverse primer) |
E. moshkovskii species | Mos-1 5' GAAACCAAGAGTTTCACAAC 3' (forward primer) Mos-2 5' CAATATAAGGCTTGGATGAT 3' (reverse primer) |
E. dispar species | ED-1 5' TCTAATTTCGATTAGAACTCT 3' (forward primer) ED-2 5' TCCCTACCTATTAGACATAGC 3' (reverse primer) |