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Table 3 Comparison of results of nested multiplex PCR (Polymerase Chain Reaction) and TechLab E. histolytica II ELISA test performed on stool specimens.

From: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples

Nested multiplex PCR result

TechLab E. histolytica II stool specimen test resulta

Total no. of specimen

 

Positive

Negative

 

E. dispar (mono infection)

0

8

8

E. histolytica (mono infection)

4

2

6

E. moshkovskii (mono infection)

0

0

0

E. dispar + E. moshkovskii (mixed)

0

2

2

E. dispar + E. histolytica (mixed)

17

1

18

E. histolytica + E. moshkovskii (mixed)

1

0

1

E. dispar+E. histolytica+E. moshkovskii (mixed)

7

3

10

Negative

0

0

0

Total

29

16

45

  1. aE. histolytica II ELISA test is designed to detect only E. histolytica specific lectin antigen in stool specimen and therefore can confirm only the presence of E. histolytica.