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Table 1 Comparison of results of nested multiplex PCR (Polymerase Chain Reaction), microscopy and culture on stool specimens.

From: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples

Nested multiplex PCR result

Microscopy and culture result a

Total no. of specimen

 

Positive by microscopy and culture

Positive by microscopy only

Positive by culture only

Negative by microscopy and culture

 

E. dispar (mono infection)

80

16

4

0

100

E. histolytica (mono infection)

12

2

1

0

15

E. moshkovskii (mono infection)

2

0

0

0

2

E. dispar + E. moshkovskii (mixed)

16

0

2

0

18

E. dispar + E. histolytica (mixed)

35

2

1

0

38

E. histolytica + E.moshkovskii (mixed)

2

0

0

0

2

E.dispar+E.histolytica+E.moshkovskii (mixed)

15

0

0

0

15

Negative

2

2

8

35

47

Total

164

22

16

35

237

  1. aMicroscopy and culture was unable to distinguish between cyst and trophozoite of E. histolytica, E. dispar and E. moshkovskii in stool specimens.