Figure 2From: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samplesDetection of E. histolytica (A), E. dispar (B) and E. moshkovskii (C) in mixed cell lysates. To 1000 cells of E. dispar and E. moshkovskii (A) or E. histolytica and E. moshkovskii or (B), E. histolytica and E. dispar (C), 1000 cells (lane 1), 100 cells (lane 2), 10 cell (lane 3), 1 cell (lane 4), 0.1 cell (lane 5), 0.01 cell (lane 6) and 0.001 cell (lane 7) of the other species were added. Amplification was done by nested multiplex PCR.Back to article page