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Figure 2 | BMC Microbiology

Figure 2

From: The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes

Figure 2

Detection of nucleosome ladders and positioning on the SL RNA gene repeat. A) Negative image of an ethidium-bromide stained agarose gel for a typical MNase digestion. Digestion of the L. tarentolae chromatin produced a characteristic ladder of DNA fragments. M = 100-bp size marker; 0 = mock treated chromatin. Increasing units of MNase are indicated, ranging from 5 – 500 units/ml. The arrowhead indicates the monosome. B) Southern blot mapping reveals strong preferential protection of an intergenic region between the T tract and the Proximal Sequence Element (PSE). DNA size marker positions are indicated on the left. Lane1, Sac II digest of the SL RNA gene in mock-treated DNA; lane 2, MNase digested chromatin. The blot was hybridized with 32P-labelled oligonucleotide probes. The letter below each blot refers to the specific oligonucleotide probe, as listed in Table 1. The probe location is indicated by the lines above the schematic. The histogram represents the ratios of the monosome signal: genomic signal.

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